Biology 1002B Lecture Notes - Lecture 21: Open Reading Frame, Dna Annotation, Asymmetric Cell Division
24 Mar 2015
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Similarity in 15. 3c are important and will be reinforced in lecture: differentiate between homology and homoplasy, mutation, duplication, rearrangement, loss mutation change in phenotype, homology: similarity due to common ancestry. Genome annotation: describing function to the agtc"s (shows what proteins) Sequence genome genome annotation align sequences determine homology. Geneticists: make algorithms that can sense certain sequences: methodology and importance of determining open reading frame (orf, protein-coding gene prediction. Repository of molecular information (dna, protein, genomes) 100,000,000 sequences deposited at ncbi today: use ncbi for sequence alignment, blast basic local alignment search tool, clustal global, sequence alignment (dna or protein) Query (what you have and send in), subject (what they give you: differences between global and local protein alignment, global alignments. They carry more information because every amino acid is made up of three nucleotides. More than one codon codes for the same amino acid: relationship between e-value and likelihood of homology, establishing homology through probability.
