Biology 1002B Lecture Notes - Lecture 9: Light-Independent Reactions, Carbon Fixation, Enzyme Kinetics
15 Feb 2016
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Lecture 9 notes (no outcomes for this lecture) anuj gupta 02/01/16. One way to measure carbon fixation is to measure photosynthesis. In a closed co2 analyzer containing chlamy (as shown in picture) if photosynthesis is working then the [co2] should drop because c is being fixed into sugar. Light response curve and co2 fixation rate: light intensity refers to photon flux. As light intensity increases more photons per unit area. The number of photons hitting the culture increases, not different wavelengths of the spectrum: if in the dark, the rate of co2 fixation is <0. It is below 0 because co2 fixation rate is negative. Co2 concentration is going up if there is no light: note that respiration is always occurring and rate of photosynthesis is 0 until there is light. As light intensity increases, the rate of co2 fixation increases. In the graph, the rate of respiration contributes to about a constant ~ -8 rate of co fixation rate.
