Biology 1202B Lecture Notes - Lecture 17: Plant Cell, Plasmid, Agrobacterium Tumefaciens
10 Mar 2016
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Can perform a variety of experiments but all rely on complementarity. Thousands to millions of molecules of probe in each spot, slightly offset each time, with many copies of that probe, allowing to hybridize many more dna. An entire transcriptome can be assessed on a single chip. >comparison identifies genes which are expressed differently, by making a cdna library from each of the two different cell types. >all common snps in a genome can be assessed on a single chip. Snps account for a large portion of the variation between individuals. Dna differences found account for a large proportion of variation between individuals. Two different samples, getting a particular sample of all of the methylated dna by using an antibody to bind. Direct manipulation of an organism"s genome using biotechnology techniques. >adding new genetic material to an organism, particularly to bacteria and prokaryotic.
