Biology 1202B Lecture Notes - Lecture 1: Reverse Transcriptase, Somatic Cell, Blood Sugar
15 Jun 2016
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Bio lecture dna technologies and application 3. Direct manipulation of an organism"s genome using biotechnology techniques: adding new genetic material to an organism, removing knock out genetic material from an organism, modifying existing genes in an organism. Using many techniques discussed previously: re digestion, ligation, cloning/expression vector. For cloning/genomic library production: don"t need to express dna, lacz (multiple re sites). Expression vectors have regulatory sequences: sequences necessary to express gene in host organism, express in e coli. Eukaryotic dna contains introns: e coli can not splice intron. Need to make cdna copy: reverse transcriptase, create cdna library, probe for gene of interest. Insulin regulates blood glucose: allows glucose transport into cells, when absent glucose builds up in blood, starves cell. Further modification to genes alter insulin properties: onset time, duration. Genes may be introduced to germ line cells or somatic cells. Insertion of you gene into mouse germ cell dna: random or targeted.
