Biology 1202B Lecture Notes - Lecture 17: Restriction Fragment Length Polymorphism, Agarose Gel Electrophoresis, Blotting Paper
14 Jul 2016
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Document Summary
Clone a line of genetically identical cells or individuals derived from a single ancestor. Dna cloning a method for producing man copies of a piece of dna. Gene of interest the gene that the researcher wants to study. Dna from 2 or more different sources that are joined together. Researcher extracts dna from cells containing the gene of interest and then cuts this dna into fragments. Each of the fragments is inserted into a plasmid. The bacterium containing the plasmids continues growing and dividing with the recombinant plasmid. The researcher then identifies which bacterium contains the plasmid carrying the gene of interest and isolate it for further study. Dna fragments produced by cutting a long dna molecule with a restriction enzyme. Short, single stranded dna fragment regions form hydrogen bonds with complementary sticky ends on any other dna molecules cut with the same enzyme.
