Biology 3326F/G Lecture Notes - Lecture 12: Coomassie Brilliant Blue, Hsf1, Hsp90
22 Apr 2016
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Document Summary
Purpose of cell stress responses (cid:120) ensure cell survival (cid:120) eliminate damaged or unwanted cells. Cells are exposed to different kinds of stress (environmental, physiological, and pathological) and develop the evolutionary conserved response, which is attributed to the heat shock proteins (hsps). Hsp 27 and hsp 70 released from nucleus for survival by heat shock response element. The transcriptional activation of hsp genes is mediated by a specific family of tfs known as heat shock factors (hsf): (cid:120) hsf1: master regulator in vertebrates and is constitutively expressed in (cid:120) most tissues and cell types. Objective of part ii: study the effects of heat shock stress (hyperthermia) on the expression of proteins in bhk-21 cells in conjunction with the state of the cytoskeleton. There are two different bhk-21 cell cultures in the study: control untreated cells growing at normal conditions, experimental cell culture exposed to the heat shock (43 c) for 90 minutes.
