Microbiology and Immunology 2500A/B Lecture Notes - Lecture 3: Double-Stranded Rna Viruses, Brainstem, Paramyxoviridae
23 Dec 2016
School
Department
Document Summary
In order for a virus to survive, it must package its genome inside a particle and then use that particle to transfer its genome from host to host. The genome contains the key information needed to initiate the viral infectious cycle. Genomes can also establish themselves in hosts to ensure their long term survival. Modern day hershey chase: use of cyanine dye - bacteriophage is mixed with cyanine dye and it binds to the viral genome, upon infection into bacterial host, the dye leaves bacteriophage and enters host interior. If you looking at a fluorescence intensity vs time graph, you will be able to see that the capsids intensity decreases with time while the cell interiors increases. Key concepts: viruses want to make mrna that is translated by host cell machinery and is read in the 5" to 3" direction. Viral genomes have to make +ssrna that is read by the host ribosomes. No virus is capable of making proteins.
