Physiology 3140A Lecture Notes - Lecture 3: Protein Kinase A, Platelet-Derived Growth Factor Receptor, Fibroblast Growth Factor Receptor

• School: Western University
• Department: Physiology
• Course: Physiology 3140A
• Professor: John Di Guglielmo

Document Summary

2 major classes of protein kinases: serine/threonine kinases, specific kinases recognize and phosphorylate serine and threonine residues that are found in specific consensus sequences, ex. Consensus sequence for protein kinase a is arg-arg-x-ser/thr: pkc, mapk, g protein-coupled receptor kinases (grks, tyrosine kinases, phosphorylate proteins at tyrosine residues found in specific consensus sequences asn-pro-x-tyr, receptor tyrosine kinases. Egfr, fgfr, pdgfr: non-receptor tyrosine kinases (ex. Gpcr structure + domain functions: transmembrane spanning domains, ligand binding, amino terminal domain, ligand binding, post-translational modification. Intracellular loop domains: g-protein coupling, regulatory protein interactions. Interaction with g protein independent signalling proteins: carboxyl-terminal domain (same functions as intracellular loop domains) Example: adrenaline stimulates glycogen breakdown in skeletal muscles. Example: gpcrs increase cytosolic ca2+ and activate pkc: plc activated downstream of active alpha subunit, plc activates pi4-5-bisphosphate into its 2nd messengers. Ip3 opens ca2+ channels causing cytosolic increase in ca2+