Physiology 3140A Lecture Notes - Lecture 1: Patch Clamp, Nuclear Pore, Western Blot
10 May 2020
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Course objective: to examine the basic principles involved in cellular physiology and survey mechanisms of intra- and inter- cellular communication of biological information, and the methods used to study these events. Course structure: online competency 3%, quizzes 12%, midterm 35%, final 50% Molecular tools: immunofluorescence, western blotting, patch clamp, rnai, pcr, mouse models. Study of how cells work together to perform functions in a living organism. Intracellular and extracellular communication, making up the organ, organ system, and the organism as a whole. Missing in this diagram: cytoskeleton (actin, mt, if), specialized organelles (lipid droplets, peroxisomes, nucleolus), cell attachments (focal adhesions) Proteins have signal sequences that separate them into different parts of the cell so they can interact. Good analogy of the intracellular environment of a cell: Proteins have a signal sequence that derive them to different parts of the cell so they can interact with different parts.
