Physiology 3140A Lecture Notes - Lecture 5: Fibronectin, Cell Migration, Collagen
10 May 2020
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Bind to a variety of components (collagen, polysaccharides, cell surface receptors, growth factors, hormones etc. ) Their major role: attach cells to ecm by cross-linking the matrix to the cell membrane. Fibronectin: needed for wound healing and cell migration; helps attach cells to other ec-components. ^ the basal lamina: incorporating proetins into the ecm. Here, perlecan is cross-linked with type iv collagen. It"s all attached to laminin in a weave. Bind the matrix as heterodimers: heterodimers are made up of alpha and beta integrins, the combination of the heterodimer tells you what the integrin will bind. Since they"re found in fibroblasts, integrins tend to form focal adhesions. Application of knowledge of the epithelium/ecm and immunofluorescence microscopy. Setting up 3d cultures of skin: reduces the need of animal testing. Note for step 3: gene transduction = genetic modification of the cell, and place it onto a basement membrane. This allows you to set up a 3e structure of skin.
