BI226 Lecture Notes - Lecture 11: Lethal Allele, Forward Genetics, Wild Type
Document Summary
Mutant flies didn"t have this cause and effect type learning. Discovered two genes: dunce and rutabaga: forward genetics, both the above genes produce proteins involved in degrading camp. Treating organism with mutagen to create mutation: saturation mutagenesis. The goal is to mutate every gene in a population of mutagenized individuals. They can be bad because once mutated, these genes are hard to clone: transposons as mutagens. Can induce mutations, but result in less mutations. Provide a dna tag" that makes cloning easier: in all mutagenesis, mutants must be crossed to a wild type individual to be sure that the only mutated gene in the genome is the one of interest. Identifying dominant or recessive mutations: dominant mutations will be expressed in the f1 generation when a mutated and a wild type individual are crossed. Usually gain of function: recessive mutations. For species that can"t self-fertilize: cross mutant with wild type, cross f1 with wild type.
