HE201 Lecture Notes - Lecture 20: Post-Translational Modification, Okazaki Fragments, Helicase
Document Summary
Advantages: cheap, fast growing, easy to maintain, stable. Advantages: post- translational modification, soluble/properly folded. Disadvantages: proteins may be insoluble/denatured, no post translational modification, expensive, unstable - chromosomal modification. If you don"t make recombinant insulin then the only insulin available is from animal sources . The solution was to make the 2 polypeptide chains separately and then artificially join them, put the chains together and put them in an oxidising environment. A = 1 - helicase means it"s an enzyme, helic-" implies helix (wind up/unwind) - eliminates 4, Digesting" will tend to be a nuclease - eliminates 3, topoisomerases correct overwinding - eliminates 2. Mice were injected with a mixture of dead virulent strain smooth (s) pneumococci and live non-virulent strain rough (r) pneumococci. They subsequently died of pneumonia and s strain bacteria were detected in their tissues, whereas mice injected with either dead s or live r alone survived.
