BIOL 3110 Lecture Notes - Horizontal Gene Transfer, Selectable Marker, Dut1
Document Summary
There is not one type of e. coli genetically. It has been altered from its wildtype form significantly and often. K12 is considered to be one of the wild type strains and is lysogenic for lambda phage and has the f" plasmid. The f factor or episome is an extrachromosomal dna that is found in some cells and can be passed on by conjugation to other cells. The plasmids we use in the lab are not like this and have been created by scientists to perform various functions. There are many different strains of e. coli mostly lab generated. Below is the genotype for one highly used strain called jm109: enda1 glnv44 thi-1 rela1 gyra96 reca1 mcrb+ (lac-proab) e14- [f" trad36 proab+ laciq lacz m15] hsdr17(rk. Le392: glnv44 supf58 (lacy1 or laczy) galk2 galt22 metb1 trpr55 hsdr514(rk. See the e. coli genotype description document on the course site as well. This is the chromosome of the e. coli strain k-12.
