BIOL 3155 Lecture Notes - Lecture 11: Orthomyxoviridae, Dete, Bovine Virus Diarrhea
Document Summary
Midterm test #2 thursday march 4th (l z vari hall b) Virus needs to use alternative strategies for gene expression to maximize the use of their small compact genome. Stranded rna viruses should be thought of and treated as mrna. Will have coding sequence and other attributes from cellular mrna. 5" end has methylguanosine attached 5" to 5" (5" cap) 5" utr and 3" utr important sequences and structures can be present within these which can influence efficiency of translation of messages, not just there as spacers. Orf is what is decoded 3 nucleotides at a time (triplet codon that converts into aa) Kozak sequence: (a/g) x x a u g g. Viral genome shown at top (only the top line with the 2 orfs represent the genome) There are 2 orfs that are encoded within the viral genome ( strand genome: polycistronic - multiple orfs on rna. Only orf1 protein is translated directly from genome.
