BIOL 4380 Lecture Notes - Lecture 3: Franz Nissl, In Situ Hybridization, Golgi'S Method
17 Feb 2018
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Document Summary
Last lectures: we have learned about how info moves from one neuron to another, and what is the nature of info that goes from one neuron to the next one. Today: some of the most common techniques used to study neural circuits in living animals. Structure architecture of the pipes, which neurons are connected to which neurons. Function of a circuit to look at the structure we"ll need to look at static and dynamic approaches. To this day, you wouldn"t know how the stain chemically does what it does. When you apply golgi stain (silver nitrate or silver salts), only random neurons stain with silver in their entirety. It allows us to view a single neuron with its dendrites and axons and everything, and doesn"t complicate the image. From the text: this method, allows the visualization of single nerve cells and their processes. It will be done in a living tissue, even in a living animal.
