CRIM 2653 Lecture Notes - Lecture 33: Atrial Tachycardia, Lipid Bilayer, Wild Type
23 May 2020
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6. 1) describe the concept of inactivation and how the presence of the inactivation state can be observed even though it is non-conducting. General notes on how the na channel gets inactivated: inactivation is a process whereby. Na channels become refractory (can"t fire another action potential until the channel has returned to a resting state) The n-termini of the channel is located in the cytosol and contains a globular domain (the ball ). To observe the presence of the inactivate state: a wild-type shaker k channel and a mutant form lacking the ball were expressed in. Xenopus oocytes (the patch clamp technique was used to monitor activity of channel): when patches were depolarized: the wild type channel opened and closed normally. The mutant opened normally but didn"t close: when a chemically synthesized globular peptide was added: the mutant channel opened and closed normally. Therefore: the ball is involved in eliciting an inactivation state after the channel had opened.
