BIO 1105 Lecture Notes - Lecture 1: Oil Immersion, Standard Ruler, Electric Light
Document Summary
Light microscope: a coordinated system of lenses arranged to produce an enlarged, focusable image of a specimen; has two/three systems- illuminating system, imaging system, and possibly view and recording system. Magnifies: increasing apparent size; accompanied by improved resolution. Resolution: the ability to distinguish two points as separate points; better resolution=sharper/crisper the image appears. Light microscope can improve resolution as much as distinguishing two points . 0000001m apart. Contrast: the amount of difference between the lightest and darkest parts of an image. Light source: comes from lightbulb located at the base of the microscope. Condenser lens: located immediately below the specimen, focuses light from the light source onto the specimen. Condenser iris diaphragm: located just below the condenser iris, a lever/ring that can be opened and closed to regulate the amount of light reaching the specimen. Imaging system: improves resolution and magnifies the image. Objective lenses: 3 or 4 lenses mounted on a revolving nosepiece.
