BIOL3040 Lecture Notes - Lecture 2: Disulfide, Immunocytochemistry, Peptide
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What are the pros and cons of using live imaging of protein tagged with gfp versus. Immunocytochemistry to visualize a protein in a cell: live imaging with gfp. Pro: you (cid:272)a(cid:374) see the (cid:272)ell"s protei(cid:374)s (cid:373)o(cid:448)i(cid:374)g arou(cid:374)d. Co(cid:374): you (cid:272)a(cid:374)"t really see the proteins well because there is so much movement. The 200+ amino acid gfp can disrupt the function or visualization of the normal protein. Manipulate the cell to express the exogenous tagged protein (not originally. Pro: you are locating at the location of the endogenous, unaltered protein (looking at the normal location. Con: by killing the cell, you cannot look at live dynamics. Killing the cell can disrupt the protein. You need an antibody that is protein specific. Monomers: can do its own thing without combining with other proteins. Heterodimers/tetradimers: form together to create a more complicated protein. Polypeptide: proteins which are formed from a chain of amino acids, side chains.
