BIOL 311 Lecture Notes - Lecture 4: Confocal Microscopy, Roger Y. Tsien, Gap Junction
Document Summary
Lecture 5 more microscopy and related topics: frap. Does it move back and forth or is it stuck in one place. Were going to add fluorescein, were looking at the cell membrane, now what this means is that the whole cell fourcesess. The next step is the use of laser (a bright laser which we can photobleach with it) we photobleach just one spot, which is no longer fluorescent. Wants to see how long it takes for that hole to be filled in: possibilities: it doesn"t fill in, or it fills in. If it fills in you can watch the rate at which it fills in (not anchored). 50% of the proteins are anchored in place: tirf. Limitations of confocal microscopy: doesn"t show body of cell. Application: tracing neurons: you have a brain, and a cell you don"t know where it goes, you want to see where it goes and track it.
