CAS BI 203 Lecture Notes - Lecture 9: Nuclear Localization Sequence, Sv40, Dna Replication

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15 Mar 2016
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For the protein immunoglobulin: they hypothesized that the 20aa that is present in rna but not in protein is responsible for the protein (immunoglobulin) to be transported out of cell (cid:1) (cid:1) (cid:1) (cid:1) Found that free ribosomes had the long version of the protein and membrane bound ribosomes had the short version of the protein. Also found that the proteins that are membrane bound ribosomes were not affected by proteinase and showed up on gel electrolysis. Sv40 and nuclear localization signal: antigen helps with dna replication through localization to the nucleus. Has a specific sequence that says bring me to the nucleus (cid:1) Find specific aa sequence (127-132) that allow nuclear entry: cut different portions of protein and see if it still goes to the nucleus. Nuclear localization signal is amino acid dependent: they can also mutate randomly along the protein and see which goes to the nucleus and which doesn"t.

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