CAS BI 525 Lecture Notes - Lecture 7: Tfeb, Tyrosine Hydroxylase, Autophagy
1 Oct 2016
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Pd continued lecture x : tfeb and alpha-syn in pd, parkin and pink1: mitochondria in pd. To look at nuclear localization: look at fluorescence in immunocytochemistry or through cell fractionation (to separate nuclear content from rest of the cell) b. If we know the genes that are the target of the transcription factor, we would look at the mrna b. i. Look up biology of transcription factor and mrna: background information for paper: c. i. Tfeb regulates transcription of autophagic genes (decrease in pd: hypothesis: does alpha-syn have anything to do with the decrease in transcription of autophagic genes when down-regulated in pd and controlled by tfeb, figure 1: slide 3 e. i. The genes controlled by tfeb are increased at day 10 (autophagic genes) e. iii. This decreases with time which is when neurodegeneration occurs (decrease in. Th tyrosine hydroxylase because less neurons) e. iv.
