CAS NE 525 Lecture Notes - Lecture 11: Membrane Potential, Axoplasmic Transport, Neurodegeneration

• School: Boston University
• Department: Neuroscience
• Course: CAS NE 525
• Professor: Lucia Pastorino

Document Summary

Sod1 aggregates trigger apoptosis by inhibiting bcl-2, a mitochondrial anti-apoptotic protein: relationship between sod1 and anti-apoptotic protein bcl-2 found on outer membrane of mitochondria. Bcl2: controls apoptosis at level of mitochondria in either stimulatory or inhibitory way. Interacts with bak and bax which form a pore which triggers mitochondrial dysfunction leading to release of cytochrome c and apoptosis. Bcl2 can bind to bak and bax aggregating them away preventing them from triggering apoptosis anti apoptosis. Bcl2 modifications prevents it from sequestering bax and bak, which leads. Sod1 associated with bcl2, but not with bax in vitro and in vivo: co-immunoprecipitation. Culture cells transfected with different mutant forms of mutants of sod1, make cell lysates, take lipid pellet (blood vessels, unbroken cells) after centrifuge and discard. Add anti body for bcl2, then add matrix (beads) that bind to bcl2 antibody which gives weight, goes to bottom. Take this part and extract bcl2 found in this part and run it in gel.