BCHM 4400 Lecture Notes - Lecture 7: Domain Analysis, Gene Ontology, Bioinformatics
11 Feb 2018
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Sequence similarity searches: identify homologous genes that are evolutionarily related in other organisms. Orthologues take similarities from different species and compare. Paralogues take similarities within same species and compare. Transferring functional annotation from previously annotated protein w sequence similarity relies on 2 assumptions: Represent evolutionarily conserved amino acid sequences w functional and structural info. Protein domain analysis helps in understanding the bio function of a gene. Ontology: terms, relationships and definitions that capture the knowledge of a domain. Term = represent controlled vocab and define concepts of domain. Provide controlled vocab to describe gene products in domains of molecular biology. Enable common understanding of model organisms and understanding between databases unify the representation of gene and gene product attributes across all species. Gene ontology annotation: based on evidence from biological experiments and/or computational analyses. A gene can be assoc with one or more go term (gene category) 3 genome browsers developed to facilitate human genome annotation.
