GEN-3000 Lecture Notes - Lecture 10: Semiconservative Replication, Human Genome, Base Pair
21 Oct 2020
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3: semiconservative replication in eukaryotes, taylor-woods-hughes (1957, showed semiconservative replication mode in eukaryotes, used root tips of vicia faba (broad bean, monitored process of replication with labeled 3h- thymidine and performed autoradiography. 4: the taylor woods hughes experiment, demonstrating the semiconservative mode of replication of d n a in the root tips of vicia faba. (a) 6: bidirectional replication of the e. coli chromosome. The thin black arrows identify the advancing replication forks. 7: the chemical reaction catalyzed by dna polymerase, during each step, a single nucleotide is added to the growing complement of the dna template using a nucleoside triphosphate as the substrate. The release of inorganic pyrophosphate drives the reaction energetically: chain elongation by dna polymerase i, occurs in 5" to 3" direction by adding one nucleotide at a time to. 9: the components making up the dna pol iii holoenzyme, as described in the text.
