BSC-2010 Lecture Notes - Lecture 7: Flagellum, Dynamic Structures, Hydrolysis

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2 Dec 2016

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Cells are the basic unit of all living organisms. Three major domains of life: bacteria, archaea, and eukarya. Protists - single cell and simple multi-cellar organisms. Cytosol semi uid, aqueous substance of the cytoplasm. Prokaryotic and eukaryotic cells di er in size and complexity. Eukaryotic cells - dna is in a membrane-enclosed nucleus. Prokaryotic cells - dna is concentrated in the nucleoid. Internal membranes compartmentalize the functions of a eukaryotic cell. Eukaryotic cells have extensive and elaborate internal membranes. Many enzymes are in or associated with membranes. Di erent local environments of compartments facilitate speci c metabolic functions. Pores in nuclear envelope allow passage of some macromolecules. Nuclear lamina proteins maintain shape of nuclear envelope. Nucleus contains most of cell"s genetic material - chromosomes. Two molecules of ribosomal rna (rrna) plus proteins. Bound ribosomes synthesize proteins; example would be digestive enzymes. Endomembrane system membranes inside (and around) a eukaryotic cell. And four other structures . (1) endoplasmic reticulum (er)

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LAB MODULE: BASIC MICROSCOPY â STUDY HELP

The information in this document is helpful in preparing for the Basic Microscopy Pre-Lab Quiz on Canvas and the Basic Microscopy quiz on Late Nite Labs.

Instructions:

â¢ Go to your Late Nite Labs course site and click on the Basic Microscopy link. The lab manual content will appear on the right side of the screen.

â¢ PRINT and READ all of the information found in the Basic Microscopy lab manual.

â¢ Use the information to answer the following questions.

Questions

â¢ Fill in the remainder of the chart.

Objective Lens	Objective Magnification	Ocular Magnification	Total Magnification
Scanning	4X	10X	40X
Low Power	10X	10X
High Power	40X	10X
Oil Immersion	100X	10X

â¢ Do all organisms consist of more than one cell?

â¢ What is the structural and functional unit of living things? In other words, what is considered the basic building block of LIFE?

â¢ What three structures are found in the cells of ALL organisms, both prokaryotic and eukaryotic? You may have to look at the pictures of the cells in the Basic Microscopy lab manual content to find all three structures. Note: plasma membrane = cell membrane

â¢ List the major differences in prokaryotic cells and eukaryotic cells? Include the location of genetic material in a eukaryotic versus prokaryotic cell.

â¢ List the organelles found in a eukaryotic cell that are not found in a prokaryotic cell. You may have to look at the pictures of the cells in the Basic Microscopy lab manual content to find all of these.

â¢ In eukaryotic cells, genetic information is found in the form of DNA. Is this true for prokaryotic cells?

â¢ Are flagella and cell walls unique to eukaryotes? Why or why not?

â¢ The __________is the part of a cell that allows the movement of substances into and out of the cell and protects the cell from its environment.

â¢ The__________is a substance that contains all of the dissolved and suspended ions and other molecules needed to keep the cell alive.

â¢ A__________is an organelle that serves as the site of photosynthesis in a eukaryotic cell.

â¢ Some organisms have cells that contain a__________that gives the cell a more structured shape and strengthens the cell by osmotic pressure.

â¢ Describe dark field microscopy.

â¢ Describe phase contrast microscopy.

â¢ Why do scientists use dark field microscopy and phase contrast microscopy when studying prokaryotic cells?

â¢ How does phase contrast microscopy help scientists visualize difficult specimens?

â¢ Fill in the remainder of the chart. The organisms studied during this lab are: Amoeba, Spirogyra, cardiac muscle, and bacteria.

Structure	Function	Organisms Studied
Plasma Membrane		Amoeba, Spirogyra, cardiac muscle, and bacteria
Cytoplasm
Nucleus	Contains the genetic material of a eukaryotic cell
Chloroplast		Spirogyra
Cell wall

1.This week, you will be conducting Gram stains of bacteria.Which of the following statements are true? (Mark all thatapply)

		Gram-negative bacteria do not stain purple because they do nottake-up crystal violet or iodine
		The peptidoglycan in the walls of Gram-positive bacteria bindsto the crystal violet stain more easily than walls of Gram-negativebacteria do
		Gram-positive bacteria have cell walls with a thickerpeptidoglycan layer than walls of Gram-negative bacteria
		Gram-negative bacteria do not stain purple, because their cellwall structure allows purple-colored iodine-dye complexes to washout of the cells more easily than the cell wall structure ofGram-positive bacteria does
		Gram-negative bacteria have an outer membrane (outside thepeptidoglycan layer in their wall) that Gram-positive bacterialack

What evidence is used to inform our understanding ofrelationships among species? (mark all that apply)

		Biochemical similarities between existing species
		Fossils
		Morphological similarities between existing species (presence ofhair, backbones, etc.)
		DNA sequences and molecular clocks

For each of the groups of organisms below, indicate whether theybelong in (A.) Archaea, (B.) Bacteria, or (C.) Eukarya

- A. B. C.	Methanogens (prokaryotes that produce methane)
- A. B. C.	Gram-positive bacteria
- A. B. C.	Fungi
- A. B. C.	Animals
- A. B. C.	Cyanobacteria
- A. B. C.	Green algae
- A. B. C.	Red algae
- A. B. C.	Amoebas
- A. B. C.	Thermophiles (prokaryotes of hydrothermal vents)
- A. B. C.	Plants
- A. B. C.	Free-living relatives of mitochondria
- A. B. C.	Single-celled organisms with a circular strand of DNA, without anuclear envelope and without peptidoglycan in their cell walls

Select all true statements about the relative diversity ofdifferent groups of organisms from among the followingstatements:

		There are more species of plants on Earth than species ofmolluscs
		There are more plant species on Earth than mammal species
		There are more species of plants on Earth than species offungi
		There are more species of fungi on Earth than mammal species
		There are more species of plants on Earth than species ofarthropods
		There are more flatworm species on Earth than mammal species

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What are the three components of the cell theory?

Of what is the cell membrane is mainly composed?

TRUE or FALSE: A prokaryotic cell has a nucleus.

TRUE or FALSE: An eukaryotic cell does not have membrane-bounded organelles.

Most organelles can be found in both in plants and animals. What three organelles are unique to plants?

What organelles can only be found in animal cells?

Which of the following contains digestive enzymes?

TRUE or FALSE: In most mammals, the DNA found in mitochondria is inherited from the mother only.

TRUE or FALSE: Flagella are not found on eukaryotic cells.

How do phospholipids interact with water to form the cell membrane?

TRUE or FALSE: All living things have a plasma membrane and a cell wall.

What is the first law of thermodynamics?

What is the example of potential energy that was given in the lecture notes?

What is the second law of thermodynamics?

What is entropy?

What is an exergonic reaction?

What is an endergonic reaction?

TRUE or FALSE: An enzyume is an organic molecule (usually a protein) that catalyzes a cellular reaction.

How does an enzyme work?

What is the region of an enzyme that binds the substrate?

TRUE or FALSE: Phagocytosis is an example of bulk transport.

TRUE or FALSE: Osmosis involves the movement of water only.

What is competitive inhibition?

What is non-competitive inhibition?

What is the energy currency of our bodies?

How do particles move in passive transport?

How do particles move in active transport?

Which type of plastid stores starch?

The largest amount of DNA is localized to the ________ in both bacteria and archaea.

Which organelle creates the lysosome?

Which component of the endomembrane system modifies proteins?

Which is the hereditary material of life?

Which organelle contains digestive enzymes to break down ingested bacteria?

In non-competitive inhibition (allosteric regulation), where does the inhibitor bind?

According to the first law of thermodynamics, energy can neither be ________ nor ________ .

BSC-2010 Lecture Notes - Lecture 7: Flagellum, Dynamic Structures, Hydrolysis

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