BIOL-K - Biology BIOL-K 322 Lecture Notes - Lecture 16: Dnaa, Helicase, Telomere
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14 Apr 2016
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Dna replicaion history: thought process and study started by watson and crick. 3 modes of replicaion: conservaive: 2 newly synthesized strands together, original helix conserved, semiconservaive: each copy has 1 parent and one new, dispersive: parental dispersed into 2 new double helices. Meselson-stahl experiment ruled out conservaive and dispersive: done with ecoli and labeled dna strands, both heavy and light n were found in each new strand (1 parent, one new) Bacterial replicaion: prokaryotes have 1 circle dna, bidirecional from 1 origin of replicaion. Polymerases can do chain synthesis, but cannot start it themselves. Replicaion forks occur at bubble, which allows for separaion when they meet up. Polymerases are very fast in prokaryotes (processive: origin sequence: rich in at (2 bonds) Eukaryoic replicaion: because of linear, muliple origin sites, replicaion much slower in eukaryoic genomes, genomes are larger, so more origins are needed to get an efecive rate.
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