BIOL 1400 Lecture Notes - Lecture 10: Helicase, Expression Vector, Dna Microarray
Document Summary
In the early 1970s, it became possible, for the first time, to isolate a selected piece of dna from the many millions of nucleotide pairs in a typical chromosome--and to replicate, sequence, and modify this dna. These technical breakthroughs are called ____________ or ____________ a novel class of nucleases that is present inside the host bacterium and cleaves dna only at certain nucleotide sequences, enzyme. ____________s restricted the transfer of dna between certain strains of bacteria, they are called restriction nucleases restriction nuclease, fragment, gel electrophoresis. After a large dna molecule is cleaved into smaller pieces with a ____________, the dna. ____________s can be separated from one another on the basis of their length by. ____________-- the same method used to separate mixtures of proteins separate the fragments on the basis of their length, one end, agarose. Gel electrophoresis is a technique used to ________________________.
