BIOL-UA 21 Lecture Notes - Lecture 7: Wild Type, Calcium Chloride, Chromosome
14 Oct 2016
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Document Summary
The break in the phosphodiester backbone is in the light blue strand, (paired to leading strand; red). Resection occurs to chew back the light blue parent strand. Strand invasion occurs where the leading strand invades the pink-light blue ligated duplex (pink and blue strands have ligated to make a parental strand in unbroken chromosome). 5" end of lagging strand (pink) ligates with the 3" end of the break of the light blue parent strand. In the picture, the branch point has moved towards the left (note length of pink area at the top), so that the branch point intersects the light blue broken strand. The free 5" end of the light blue pairs with the dark blue strand. Holliday junction is resolved between light blue and pink, and once the ends are ligated, the replication fork is xed: generation of antibody diversity:
