BIOL-UA 21 Lecture Notes - Lecture 11: Histone H3, Hybridization Probe, Sister Chromatids
18 Mar 2016
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Document Summary
Chromosomes carry the genetic information into daughter cells. Karyotype numbers, size and shape of chromosomes. Eg. giemsa, a permanent dna dye that binds to dna after the chromosomes are subjected to mild heat or proteolysis; this yields g bands. Each chromosomes has its own giemsa band pattern: Eg: fish (fluorescence in situ hybridization), based on hybridization of fluorescence labeled probes specific to different regions of the genome. Probes = short dna fragments that you generate from. You can add labeled nucleotides with a detection probe, usually a fluorescent dye in the dna. When you take dna probe, you denature it, then hybridize it to the mitotic chromosome, it will hybridize to regions where it"s complementary to it. Take that and look under fluorescent micrograph and will give you an image of the karyotypes. X shape chromosome structure, two sister chromatids joined by centromeres with ends at the telomere. First level of compaction = due to nucleosomes.
