BIOLOGY 1113 Lecture Notes - Lecture 44: Expressed Sequence Tag, Dna Sequencing, Dna Annotation
2 Dec 2018
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All credit goes to freeman biology 6th edition 2017. Biotechnology- engineering of genes/cells/organisms for research(adding/editing specific genes, gm crops, curing disease, determining dna sequences(evolutionary relationships, modified proteins/enzymes)). Gene sequences allow us to infer amino acid sequence(function), and different alleles(disease), evolutionary relationships. Dideoxy sequencing- technique determining nucleotide sequence of dna(uses ddntps(no 3"oh), which terminate dna replication)(long sequences), end of every strand contains fluorescent label(tells which base is present). Next gen sequencing- dna sequencing technologies that are more rapid/cheaper than dideoxy sequencing(millions of dna in single run). Very short pieces of dna, hard to piece together. Shotgun sequencing- sequencing genomes based on breaking genome into small pieces, sequencing each piece separately, figuring out how pieces are connected(genome assembly, many copies of genome). Bioinformatics- computer science branch concerned w/ managing, interpreting, and analyzing biological info(dna/amino acid sequences). Genome annotation- analyzing genome sequence and identifying features such as genes, regulatory sequences/splice sites. Gene- segment of dna regulating production of and codes for functional.
