BICH 410 Lecture Notes - Lecture 7: Elution, Ion Exchange, Gel Electrophoresis
Document Summary
Proteins must be puri ed in order to be used for their function. Protein puri cation process: allows for the separation of different types of proteins. Fractionation- separation of components of a mix of molecules based on physical and chemical properties. Quantitation - detect and quantify the protein in the presence of other proteins at teach step of the puri cation procedure. Measuring the uv of a protein compound can help gure out the relative size (aromatic amino acids absorb in the uv) Use low salt concentration to precipitate unwanted proteins. Use higher salt concentration to precipitate target protein. Ion exchange removes proteins with higher solubility removes protein with lower solubility. Smaller molecules will be able to enter the beads. Possibility of losing some of the sample because of dilutions. Close molecular weights would be hard to measure. Only depend on size rather than charge since all the molecules are negative.