BIL 255 Lecture Notes - Lecture 2: Green Fluorescent Protein, Osamu Shimomura, Roger Y. Tsien
12 Mar 2018
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Document Summary
Chemical fluorophores can be used in live and fix specimens: small molecules that absorb and emit light at different wavelengths, examples: cy, rhodamine, fluorescein, dapi, alexa, can be covalently attached to other molecules (protein inhibitors, antibodies, etc. ) Analogize the tem and the sem to light microscopes: answer: a. What are cells: biochemist approach, cell breakage, biodetergant to lyse membrane, grind with plunger, force cell through small hole using high pressure, break cells with high frequency sound, cell fractionation through centrifugation, differential centrifugation, velocity centrifugation, equilibrium centrifugation. Shared characteristics of model organisms: chemistry, same elements, same type of molecules, biochemistry, same type of catalytic reactions", biomechanics, same type of structural support units, motors, etc. What makes a model organism: able to grow in lab, short generation time/many progeny, unique feature appropriate to research, a community, genome sequence, some clear scientific advantage.
