LIFESCI 3 Lecture Notes - Lecture 8: Dna Replication, Semiconservative Replication, Thymine

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Ls 3 - lecture #8 - dna replication. For the first exam, know process of previously discussed experiments, how it was carried out, what the results were, and what the significance of the results were. Dispersive has single bands, but become lighter with each round of dna replication because the ratio will decrease. For semiconservative note that there are different densities among daughter dna. Clicker question: suppose you are provided with an actively dividing culture of e. coli bacteria to which radioactive thymine has been added. Dna in one daughter cell would be radioactive but not the other. Dna in both daughter cells would be radioactive. Neither of the daughter cells would be radioactive. Answer: dna in both daughter cells would be radioactive. Through the semiconservative model, because each daughter cell is half parental, half new. The first choice would have been correct if the conservative model were true.

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