BICD 100 Lecture Notes - Lecture 12: Genetic Screen, Reverse Genetics, Point Mutation
29 Oct 2016
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Bicd100 lecture 11 forward genetic screens answer: substitutions: we don"t know the functional change to gene/protein, we only know what the mutagen is going to do, enu causes substituations. What is we wanted to do a forward genetic screen in worms. Xo male answer: eliminate cross #2, no need to cross with another wt. Using dna polymorphisms in human genetics: pedigree analysis, association studies. Reverse genetics: manipulating the genome: putting things in, taking things out. If dna polymorphism is unlinked to your mutation, how oftern will they co-segregate: how often will the dna polymorphism be homozygous in homozygous mutant embryos. Only 4 possibilities: 2 heterozygous, dominant homo, recessive mutant homo. If unlinked, they will cosegregate of the time. (1) the existence together of many forms of dna sequences at a locus within the population. (2) a discontinuous genetic variation that results in different forms or types of individuals among the members of a single species.
