BICD 100 Lecture Notes - Lecture 11: Plasmid, Mendelian Inheritance, Knockout Mouse
1 Nov 2016
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Document Summary
Genbank: largest publicly available database of dna sequences. Blast: basic local alignment search: software application used to compare a segment of genomic dna to sequences throughout major databases, e value: expect value. Based on number of matching sequences in database expected by chance. Identification of genes: can identify location of introns depending on different splice sites. Protein-coding regions: there are several identifiable features indicating that the sequence contains a protein-coding gene. Homologous genes: genes that are evolutionarily related, similarity searches are able to identify homologous genes, orthologs: genes from different species thought to have descended from common ancestor, paralogs: homologous genes in same species. Protein domains and motifs: when gene sequence used to predict polypeptide sequence, polypeptide analyzed for specific protein domains and motifs. Protein domains: ion channels, membrane spanning regions, secretion, and export signals. Motifs: helix-turn-helix, leucine zipper, or zinc-finger motifs.
