BICD 100 Lecture Notes - Lecture 11: Leucine Zipper, Genbank, Plasmid
19 Dec 2016
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Can blast search nucleotides or protein sequences. Protein coding regions: annotation of sequence (figure a) reveals several identifiable features indicating that the sequence contains a protein-coding gene. Three exons (figure b); two unshaded areas between exons represent introns; later spliced out during mrna processing: a single strand has 3 different reading frames (so 6 total for 2 strands) The strand depends on which direction we try to read it in (cid:894)do(cid:374)"t (cid:374)e(cid:272)essaril(cid:455) k(cid:374)o(cid:449) which strand is coding and which strand is template) Predicting gene function: functional categories assigned for human genes on basis of. Comparison to known genes and predicted protein sequences from other species. Predictions based on annotation and analysis of protein functional domains and motifs. Sterotypical binding sequences, something that you would expect to see in a protei(cid:374) that"s e(cid:373)(cid:271)edded i(cid:374) a plas(cid:373)a (cid:373)e(cid:373)(cid:271)ra(cid:374)e. Can give a clue as to what it does.
