BIOL 2003 Lecture Notes - Lecture 1: Polyclonal Antibodies, Epitope, Hybridoma Technology

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11 Oct 2018
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Adaptive immune response two molecules: immunoglobulins and t-cell antigen receptors. Function: antibodies, recognize epitope of immunogen (an antigen) Light and heavy chain connected by covalent and non-covalent bonds. Antibodies and antigens non-covalent bonds: hydrogen, electrostatic bonds, van der waals and hydrophobic forces. Cross reactivity: some regions in the epitope of an antigen are share by another antigen. Produced from single b-cell type very specific to one epitope. Can"t recognize the antigen if it undergoes chemical changes or degradation. In lab, produced from hybridomas cells, by fusing immortal myeloma cancer cells with spleen cells from a mouse that has been immunized against a desired antigen: polyclonal: In lab, produced by immunizing a suitable mamma with a solution containing a specific antigen. An immune response is induced and the b- cells then produce a mixture of antibodies specific to this antigen. Polyclonal antibodies can then be purified from the mammal"s serum. Purpose: determine localization of proteins within a cell.

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