BIOC 3110 Lecture 36: RNA synthesis and Regulation in Prokaryotes
Document Summary
Lecture 36: rna synthesis and regulation in bacteria. Rnap works by reading the template dna strand (aka non-coding/anti- sense strand(cid:524) in the 3" 5" direction, and produces mrna in the 5" 3" direction. In ecoli, rnap transcribes all rna types, not the case in eukaryotes. Promoter is around -35 -10 region; place where rnap binds and recognize gene to which it is going to transcribe. Omega subunit binds and recognizes the promoter region on the dna template strand. Holoenzyme-term referring to the initiating enzyme that includes all subunits listed above. For most genes in ecoli, this is the rate limiting steps, so this holoenzyme binding to the promoter region is highly regulated (ex. Once rnap recognizes the region, it transitions from: what subunits are found in the elongating rna? begins to synthesize closed to open, as the coding and template dna strand spread apart to allow for transcription. Elongating core enzyme-term used to describe the portions of.