1. (20pts) LetΓ’ΒΒs consider how restrictive enzymes work. Look the two samples of DNA shown below:
DNA Sample #1
5Γ’ΒΒΓ’ΒΒ T C C A G T G A T C T C G A A T T C G C T A G T A A C G T T C G A T
3Γ’ΒΒΓ’ΒΒ A G G T C A C T A G A G C T T A A G C G A T C A T T G C A A G C T A
DNA Sample #2
5Γ’ΒΒΓ’ΒΒ T C A T C G A A T T C C T G G A A T C A G C A G A A T T C G C A T A
3Γ’ΒΒΓ’ΒΒ A G T A G C T T A A G G A C C T T A G T C G T C T T A A G C G T A T
If both samples are treated with the restriction enzyme EcoRI [recognition sequence GΓ’ΒΒAATTC] then indicate the number of fragments and the size of each fragment from each sample of DNA. List the fragment sizes in order of largest to smallest.
Sample # 1 Sample # 2
# of fragments:________ # of fragments:________
Sizes of fragments (largeΓ small): Sizes of fragments (largeΓ small):
(20pts) Read the protocol for Lab 8B.
What is supercoiled DNA
Why would it migrate further into an agarose gel if itΓ’ΒΒs uncut plasmid DNA?
What causes this type of DNA to form?
Is this likely to occur to our isolated plasmid DNA?