CHEM 271 Lecture Notes - Lecture 5: Dissociation Constant, Dextran, Unified Atomic Mass Unit
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Due: december 1st, 2021: gel filtration chromatography. Vo: initial volume eluted of blue dextran = 37. 36 ml. Ve of blue dextran: 37. 36 min x (1 ml/min) = 37. 36 ml. Ve of catalase: 60. 85 min x (1 ml/min) = 60. 85 ml. Ve of bovine serum albumin: 68. 18 min x (1ml/min) = 68. 18 ml. Ve of cytochrome c: 85. 24 min x (1ml/min) = 85. 24 ml. Ve of vitamin b12: 121. 4 min x (1ml/min) = 121. 4 ml. Internal volume (vi) of the column: vi = vs- vo. Vi = volume of vitamin b12 volume of blue dextran. Distribution coefficient (kd): kd = (ve - vo) / vi. Kd blue dextran = (37. 36 ml 37. 36 ml) / 84. 04 ml = 0. Kd catalase = (60. 85 ml 37. 36 ml)/ 84. 04 = 0. 2795. Kd bsa = (68. 18 ml -37. 36 ml) / 84. 04 = 0. 3667. Kd cytochrome c = (85. 84 ml -37. 36 ml) / 84. 04 = 0. 5769.
