BIOL 201 Lecture Notes - Rna Polymerase Ii, Cis-Regulatory Element, Tata Box
Document Summary
Lecture 19: regulatory sequences in rna polymerase ii promoters. The tata box is not the only proximal cis-acting regulatory element driving transcriptional initiation: tata box may be accompanied by or substituted by other sequences. Transcription occurs in both directions off some promoters tfiid may impart directionality to rna pol ii: both in sense and antisense direction, particularly evident on promoters without tata boxes (made up of cpg islands: correspond to sequence. C-phosphate-g: seen in metabolic genes, genes involved in general cellular housekeeping, at cpg-rich promoters bidirectionality of transcription occurs, don"t know why, but the sense transcript tends to be much more efficient. Recombinant dna technology to better understand and analyze enhancers: dna cloning in a plasmid vector permits amplification of a dna fragment, bacteria transformation. Interested in a particular region of a promoter: take region of interest and eliminate chunks from the 5" end using pcr.
