ANSC 3080 Lecture : Laboratory 1 - Worksheet
Document Summary
Determining the presence or concentration of hormones in body fluids. After completing this laboratory exercise, you should be able to: Understand the importance of knowing the concentration of hormones in biological samples for areas such as research, clinical applications, and herd management decisions. Explain, illustrate and execute an elisa and a lfia for quantification of hormones in biological samples. Signaling molecules that modulate the activity of the target tissue. Provides important insight into normal (estrus, preg,) and disease conditions. Feces - wild species but not routinely used for domestic species. Hands-on activities: using competitive lfia to detect the presence of p4 in bovine milk samples. The lfia is a paper-based platform for the detection of hormones in body fluids, where the sample is placed on a test device and the results are displayed within minutes. A typical lfia strip consists of overlapping membranes that are mounted on a backing card for better stability and handling.
