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20 Dec 2018

Explain the workflow of sequencing a protein using the Sanger method or Edman degradation?

Compare and contrast older methods of protein sequencing with the current method of Mass Spectrometry?

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Elin Hessel
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21 Dec 2018

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Related questions

. Next Generation DNA Sequencing methods have dominated the study of microbial diversity in the last 12 years. Compare and contrast the Sanger vs the Illumina approach to DNA sequencing. First explain how each approach works, first the Sanger method then Illumina. Then, compare those two methods based on various Explain the principles of NGS its advantages and disadvantages as a tool for the characterization of microbial diversity.

In the laboratory, you are studying TrbL, a 70 kD protein that causes tribbles to be furry. You isolate a 70 kD protein that you believe to be TrbL using gel filtration chromatography. When you run your sample on an SDS-PAGE gel and stain with Coomassie, you observe a single band at 70 kD. To determine the sequence of the N- terminal region of the protein, you carry out several rounds of the Edman degradation. Unfortunately, the first round suggests that you have both alanine and tyrosine present. The second round tells you that you have both tryptophan and valine present. In fact, each of the rounds of the Edman degradation suggest that two amino acids are present.

a. Assuming that your protein sequencing apparatus is functioning correctly, suggest a simple explanation for your result.

b. Give a simple experiment that will allow you to test your answer to part a.

c. How would you test your protein sequencing apparatus to be certain that it is working correctly and that your difficulties are specifically associated with your sample of 70 kD protein?

Please answer all 3 parts and give an explanation. Thank you very much! Much appreciated!

PLEASE ANSWER ALL PARTS FULLY!

How was liquid hybridization used to compare species relatedness? What is the purpose/significance of Driver versus Tracer DNA? What is Subtractive Hybridization, how/why would you use this technique?

Compare Maxam-Gilbert and Sanger DNA sequencing methods. What are the more descriptive names for these techniques? How are the techniques similar? different? What reagents are used in each reaction? How are the results read? Do you get the sequence of the molecule you started with or the complementary molecule? How does fluorescent cycle sequencing differ from the original Sanger Method?

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