. A yeast cell has a temperature sensitive mutation in the gene that is involved in mating between yeasts of opposite mating types. The yeast cells do not mate at 37 degrees (restrictive temperature) but do mate at 30 degrees (permissive temperature). Would you expect the introduction of a gene from mice into this mutation to facilitate mating at the 37 degrees? Why?

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7 Jun 2023

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7 Jun 2023

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Hello,

I am having a hard time understanding what temperature-sensitivemutation is about? I understand that the wild type phenotype isobserved at permissive temperature and the mutation can only beseen at the restrictive temperature. However no one is able toexplain to me what specific change in the amino acid sequenceaccounts for the temperature sensitive phenotype. What happens atthe molecular level?

I've been asked the following question during a recent test andI didnt even know how to answer it:

I have isolated a series of mutant strains. Below is adescription of the mutant phenotypes. Each mutant strain contains amutation in a single gene. Please describe what gene is defectiveand why.

A temperature sensitive strain of yeast grow slowly at thepermissive temperature of 30 C but does not grow at 37 C. At 30 C,proteins are synthesized but at a slower rate. However, at 37 Cprotein synthesis ceases after 30 minutes.

whitealligator157

2. A human gene X has been identified which, when mutated, causes a genetic illness. The sequence of gene X has been determined, but the function of the gene is not known. Sequence analysis reveals that the gene has a high degree of homology with a yeast gene of known function. You would like to determine if human gene X has the same function as the yeast gene. You have obtained a yeast mutant which has a deletion mutation in this gene. The phenotype of the yeast mutant is that it grows normally at 30Â°C but cannot grow at the elevated temperature of 37Â°C. You hypothesize that if the human gene has the same function as the yeast gene, then expressing the human gene in the yeast mutant will rescue the mutant phenotype, enabling it to grow at 37Â°C. Address the following questions regarding the steps you will take to test the hypothesis:

a. You have a source of total human cDNA. How will you clone the specific cDNA for gene X? (Do not just name the technique that you will use, but list the specific steps).
b. You would like to clone the cDNA of X into a plasmid that can be amplified in bacteria and from which reasonable yields of protein can be obtained by expression in yeast. List all the features that this plasmid must have.

Please explain your answer! Thanks

Your boss gives you a dish ofmammalian cells in culture. She tells you these cellsare unusual because they have a temperature-sensitive, dominantmutation in an unknown gene. Shifting cells with thismutation to the restrictive temperature causes immediateinactivation of kinesin-5, a plus-end directed interpolarmicrotubule motor. Your boss tells you the dominantnegative mutation has no other effect on your cells and that yourcells are replicating as an asynchronous population (i.e. cells areat random stage of the cell cycle). You place the dishof cells at the restrictive temperature around 9 pm. Thenext morning you retrieve the dish and examine the cells under themicroscope. Which of the following is most likely to be observed?

(1 point)

All of the cells are arrested in G1.

Approximately half of the cellsappear arrested in mitosis.

Approximately half of the cells arearrested in S-phase.

Approximately half of the cells arearrested in G0.

All cells appear normal but are indifferent stages of the cell cycle.