1.You are studying the worldâs smallest enzyme which has the amino acid sequence of:
Met Ala Ser Met Tyr Ala Glu
You know that activity of this enzyme requires phosphorylation. You wish to design an inactive mutant form of this enzyme that can never be phosphorylated. You will use site-directed mutagenesis to alter the DNA sequence of this protein, resulting in a mutant protein.
Additional information: The most common resides to be phosphorylates are serine, threonine and tyrosine. In theory, these can be replaced with any other amino acid except for glutamic and aspartic acid, the shape and charge of a phosphorylated serine and aspartic acid are similar. Substitution with one of these amino acids would create a protein that looked like it was always phosphorylated (phosphomimic). Alanine is one of the most common substitutions, but others are possible and sometimes preferable to preserve other characteristics of the amino acid.
1. Write out the resulting amino acid sequence corresponding to your designed mutant protein here:
1.You are studying the worldâs smallest enzyme which has the amino acid sequence of:
Met Ala Ser Met Tyr Ala Glu
You know that activity of this enzyme requires phosphorylation. You wish to design an inactive mutant form of this enzyme that can never be phosphorylated. You will use site-directed mutagenesis to alter the DNA sequence of this protein, resulting in a mutant protein.
Additional information: The most common resides to be phosphorylates are serine, threonine and tyrosine. In theory, these can be replaced with any other amino acid except for glutamic and aspartic acid, the shape and charge of a phosphorylated serine and aspartic acid are similar. Substitution with one of these amino acids would create a protein that looked like it was always phosphorylated (phosphomimic). Alanine is one of the most common substitutions, but others are possible and sometimes preferable to preserve other characteristics of the amino acid.
1. Write out the resulting amino acid sequence corresponding to your designed mutant protein here:
