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Biology
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14 Sep 2018

The function of the promoter region of a gene is:

A) to encode an amino acid sequence

B) to identify which strand is the template strand and where to start transcribing

C) to provide a binding site for the ribosome

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Related questions

1) The following DNA strand is a template strand of a prokaryotic gene.Transcription start siteis indicated by a bold “G”.

a) Underline the promoter region of this gene by a dotted line.

b)Underscore the Pribnow box in the promoter region. What is the function of the Pribnow box?

c) Deduce the nucleotide sequence of mRNA for this gene.

d) Underscore the leader sequence in mRNA and box the initiation codon. How many amino

acids does this mRNA code for? What is the sequence of the codons in this mRNA?

e) Show 5’ and 3’ ends of the template strand and mRNA.

f) What are the -10 and +10 base pairs of this gene?

CCCTCCGTCGCTATAATGAAGTCGGAGACGGATGTACCGCGGATAA

Order the following choices to examine the process of transcription.

- RNA polymerase moves along the template strand of DNA adding complementary RNA nucleotides, extending the mRNA.

-RNA polymerase binds at the promoter region just upstream from the gene.

-RNA polymerase unwinds the DNA at the promoter site and begins to transcribe the template strand of DNA.

-Transcription continues until a termination site in the DNA is reached.

-The mRNA transcript is released.

2) For the hypothetical DNA sequence/gene below mark the start codon and the stop codon of the gene. TSS->

5’CATAAA| GCAAACAGGAGATGGGCCCCAAAAGCCATGAATGGGAGCGCGTATAATCATGATTTATAATCTAGG TATCTCACTGA3’

3’GTATTT| CTTTTGTCCTCTACCCGGGGTTTTCGGTACTTACCCTCGCGCATATTAGTACTAAATATTAGATCC ATAGAGTGACT5’

Terminator sequence

Promoter sequence

TSS-> transcription start site and direction of transcription

a) Which strand of the DNA shown above, the top or the bottom, is the template strand?

b) What is the sequence of the mRNA produced from this gene? Label the 5’ and 3’ ends.

c) What is the sequence of the protein produced from the mRNA in (b)? Label the N and C termini.

d) Generate two examples each that result in a silent mutation, a missense mutation, a nonsense mutation, and a frameshift mutation for the DNA sequence above. Show the respective amino acids below each codon. (Courier font is a font typically used for sequences.)

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