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9 Sep 2018

Calculate the proton motive force from the membrane potential and pH gradient across the inner mitochondrial membrane.

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Keith LeannonLv2

10 Sep 2018

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A)Peter Mitchell and colleagues determined that the total proton motive force across the inner mitochondrial membrane at 37 degree celsius was 230mV, of which 70% was due to the electrical potential. What was the pH gradient across the membrane?

1. Compared to the generation of ATP by oxidative phosphorylation, what is a major difference in mechanism of ATP generation by substrate-level phosphorylation in glycolysis?

A. A proton gradient is not involved

B. The ATP produced has less energy content because oxygen was not involved.

C. Substrate level phosphorylation occurs in the inter-mitochondrial space

D. One can't generalize without knowing the substrate.

2. Which of the following statements is true?

A. Because the electrons in NADH are at a higher energy than the electrons in reduced ubiquinone, the NADH dehydrogenase complex can pump more protons than can the cytochrome b-c1 complex.

B.The pH in the mitochondrial matrix is higher than the pH in the intermembrane space.

C. The proton concentration gradient and the membrane potential across the inner mitochondrial membrane tend to work against each other in driving protons from the intermembrane space into the matrix.

D. The difference in proton concentration across the inner mitochondrial membrane has a much larger effect than the membrane potential on the total proton-motive force.

QUESTION 1

Describe in detail the route of electron flow from NADH to oxygen that establishes a proton gradient across the inner mitochondrial membrane.

QUESTION 2

When the F₁ portion of the ATP synthase complex is removed from the mitochondrial membrane and studied in solution, it functions as an ATPase. Why does it not function as an ATP synthase?

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Calculate the proton motive force from the membrane potential and pH gradient across the inner mitochondrial membrane.

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