BIO 1140 Chapter Notes - Chapter 6: Cytoskeleton, Ubiquitin, Phospholipid Scramblase

• School: University of Ottawa
• Department: Biology
• Course: BIO 1140
• Professor: Caroline Petit- Turcotte

Document Summary

Mitochondria are responsible for: metabolism, response to stress, ca storage, cell death. Used to isolate mitochondria in order to study it. Separates components by spinning: variety of methods with different objectives. Examples: diff. spin speeds/ using buffers to change density of specific components. Spin using different technique to isolate for target component. Use e(cid:374)e(cid:396)g(cid:455) to spi(cid:374) (cid:373)oto(cid:396) i(cid:374) o(cid:396)de(cid:396) to phospho(cid:396)(cid:455)late adp atp. Can work backwards: use atp to contribute to proton gradient (pumps h+ out) Different functions located in diff. areas of mitochondria. On folds of inner membrane (between matrix and inner membrane space) Folding of inner membrane (cristae)=more sa=more protein complex to build etc. Larger, more efficient h gradient to power f-type atpase. Important for: release neurotransmitters, cellular signalling, bones, muscle contractions, enzymatic cofactor for cellular functions, metabolism. Toxic to (cid:272)ells: must (cid:271)e sto(cid:396)ed/ hidde(cid:374) , [ ] (cid:449)ithi(cid:374) (cid:272)ell (cid:373)ust (cid:271)e (cid:396)egulated. [ca2+] must be kept such that: ca2+ readily available but not free in cytosol.