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I need some help finding a few interesting scientific articles where they used qpcr / ( real time PCR) Maybe 3-10 links to look at would be extremely appreciated. please include links to them and the paper need to not be on qPCR just a paper where they used it. when I search for it I just find boring papers mostly on qPCR ... I would like some help finding something a little more interesting

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Reid Wolff
Reid WolffLv2
28 Sep 2019

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What would be really interesting is if there had been someexperiments or simulations carried out during a very long time,plausibly allowing for extremely low-probability structures toform. I doesn't matter whether this simulation/experiment has beencarried out in a lab or as a computer simulation. There is ofcourse the interesting but old "Conway's Game of Life" simulation,but according to my search efforts nobody has really tested it on avery large scale and in 3D.

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I JUST NEED THE ANSWER FOR PART 2

Part I. You are interested in sequence conservation and function of the androgen receptor (AR) across vertebrate species. The first thing you will want to do is collect protein sequences of the AR from these selected species: Homo sapiens (human), Pan troglodytes (chimp), Mus muculus (mouse), Canis familiaris (dog), Gallus gallus (chicken), Danio rerio (zebrafish). To find these protein sequences I set up the search of the protein database with the search terms "androgen receptor" "AR" and then the appropriate genus and species. Choose the hit with the longest amino acid sequence, likely to be the complete sequence of the protein.

Prepare a summary of your comparison as if you were making a presentation to a class (OK, a ClustalOmega with some text as the minimum). Be sure to include

1. An analysis of the molecular evolution of this gene across vertebrate species. What is the variation in sequence by region of the protein likely to mean?

2. Anything intriguing about particular "poly-amino-acid repeats" you see? That is, the familiar "poly-Q" that is the site of disease alleles in huntingtin, or any other case of a repeating amino acid? (I personally noted 3 such regions; this is open-ended but your score on this section will depend on the quality of your assessment and presentation)

Part II. Clearly, there is a region of the AR that is highly conserved across vertebrates! Does this encode one or more functional domains that might also be conserved in female hormone receptors? (In particular, receptors for the female sex hormones estrogen and/or progesterone.) In other words, are there domain features that maybe overlap in both male and female hormone receptors?!

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Might DMEM alone (without FBS) provide a little more impetus tostay attached during the assay? What would the effect be ofincreasing the amount of FBS to say 15 or 20% during culturing -would that promote stronger attachment? I'm a little hesitant tocoat the wells with poly-D-lysine, as my readout is on afluorescent imager, and I'm worried it would substantially increasebackground. I don't really have enough time to re-select the cellsfor adherent growth (although if I'd known about these issues frommy predecessor 3 or 4 months ago I would have started rightaway...), so are there any other tricks I can try in the meantime?Are there any media supplements I could use to promote adherinexpression or something like that? Any tips or advice at all wouldbe appreciated.

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