The base sequences of 2 DNA clones are below. The base sequenceof only 1 strand of each clone is shown. One clone is a cDNA cloneand the other is a genomic DNA clone. Both clones code for the samepolypeptide encoded in the nuclear genome of dogs. Annotate the clones and answer the following questions:

Clone 1:3

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Elin HesselLv2

28 Sep 2019

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1. We can construct our gene library by cloning the PstI fragments into either plasmid pBR322 or phage lambda. Which one should we choose and why?

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You cut pUC18 vector DNAwith SmaI. You cut the insert DNA with EcoRI. Can you clone theinsert into this vector?

Yes, without any further modification of the insert.

Yes, only after making the insert DNA blunt-ended by anenzyme.

Yes, only after making the vector DNA sticky using anexonuclease.

You need to separate ssDNA molecules that are differentfrom each other by one or a few nucleotides in size. You shoulduse________ gel.

1-2% native agarose

2-3% denaturing agarose

3-4% native polyacrylamide

5-6% denaturing polyacrylamide gel

2% agarose pulsed field